Hertwig's epithelial root sheath cells do not transform into cementoblasts in rat molar cementogenesis

• Yamamoto, Tsuneyuki

• Takahashi, Shigeru

抄録

It is generally accepted that cementoblasts originate in the process of differentiation of mesenchymal cells of the dental follicle. Recently, a different hypothesis for the origin of cementoblasts has been proposed. Hertwig's epithelial root sheath cells undergo the epithelial-mesenchymal transformation to differentiate into cementoblasts. To elucidate whether the epithelial-mesenchymal transformation occurs in the epithelial sheath, developing rat molars were examined by keratin-vimentin and Runx2 (runt-related transcription factor 2)-keratin double immunostaining. In both acellular and cellular cementogenesis, epithelial sheath and epithelial cells derived from the epithelial sheath expressed keratin, but did not express vimentin or Runx2. Dental follicle cells and cementoblasts, however, expressed vimentin and Runx2, but did not express keratin. No cells showed coexisting keratin-vimentin or Runx2-keratin staining. These findings suggest that there is no intermediate phenotype transforming epithelial to mesenchymal cells, and that epithelial sheath cells do not generate mineralized tissue. This study concludes that the epithelial-mesenchymal transformation does not occur in Hertwig's epithelial root sheath in rat acellular or cellular cementogenesis and that the dental follicle is the origin of cementoblasts, as has been proposed in the original hypothesis.

収録刊行物

• Annals of Anatomy

  Annals of Anatomy 191 (6), 547-555, 2009-11-20

  Elsevier

キーワード

• Cementoblasts
• Hertwig's epithelial root sheath
• Epithelial-mesenchymal transformation
• Keratin
• Runx2
• Vimentin
• Rat molars

詳細情報

• CRID: 1050001339002139648
• NII論文ID: 120001648579
• NII書誌ID: AA10833187
• HANDLE: 2115/39726
• ISSN: 09409602
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles