Nesfatin-1 evokes Ca2+ signaling in isolated vagal afferent neurons via Ca2+ influx through N-type channels

• Iwasaki, Yusaku

• Nakabayashi, Hajime

• Kakei, Masafumi

• Shimizu, Hiroyuki

• Mori, Masatomo

• Yada, Toshihiko

抄録

金沢大学医薬保健研究域医学系

Nesfatin-1, processed from nucleobindin 2, is an anorexigenic peptide expressed in the brain and several peripheral tissues including the stomach and pancreas. Peripheral, as well as intracerebroventricular, administration of nesfatin-1 suppresses feeding behavior, though underlying mechanisms are unknown. In this study, we examined effects of nesfatin-1 on cytosolic Ca2+ concentration ([Ca2+]i) in the neurons isolated from the vagal afferent nodose ganglion of mice. Nesfatin-1 at 10-10-10-8 M increased [Ca2+]i in the isolated neurons in a concentration-dependent manner, and at 10-8 M it increased [Ca2+]i in 33 out of 263 (12.5%) neurons. These responses were inhibited under Ca2+-free conditions and by N-type Ca2+ channel blocker, ω-conotoxin GVIA. All the nesfatin-1-responsive neurons also exhibited [Ca2+]i responses to capsaicin and cholecystokinin-8. These results provide direct evidence that nesfatin-1 activates vagal afferent neurons by stimulating Ca2+ influx through N-type channels, demonstrating the machinery through which peripheral nesfatin-1 can convey signals to the brain. © 2009 Elsevier Inc. All rights reserved.

収録刊行物

• Biochemical and Biophysical Research Communications

  Biochemical and Biophysical Research Communications 390 (3), 958-962, 2009-12-18

  Elsevier

キーワード

• Cholecystokinin
• Cytosolic Ca2+
• Feeding
• N-type Ca2+ channel
• Nesfatin-1
• Nodose ganglion

詳細情報

• CRID: 1050282810930513152
• NII論文ID: 120001871396
• NII書誌ID: AA00564395
• ISSN: 0006291X
• Web Site: http://hdl.handle.net/2297/20509
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles