Interleukin-6 upregulates the expression of PMP22 in cultured rat Schwann cells via a JAK2-dependent pathway

• Ito, Takaaki

• Ikeda, Kazuo

• Tomita, Katsuro

• Yokoyama, Shigeru

抄録

金沢大学医薬保健研究域医学系

The interleukin-6 (IL-6) family of cytokines is thought to be involved in the development and regeneration of peripheral nerves; however, their roles in myelination remain unclear. In this study, we examined the effects of IL-6 on the expression of genes for compact myelin proteins using Schwann cell cultures prepared by multiple explantation of adult rat sciatic nerves. In semi-quantitative reverse transcription-polymerase chain reaction analysis, stimulation of Schwann cells with IL-6 significantly increased the mRNA level of peripheral myelin protein 22 (PMP22), but not those of myelin protein zero and myelin basic protein. The increase in PMP22 mRNA was markedly suppressed by AG490, a Janus kinase 2 (JAK2) inhibitor, but not significantly by PD098059, a mitogen-activated protein kinase inhibitor. Immunocytochemical staining revealed that IL-6 enhanced immunoreactivities for the phosphorylated forms of both JAK2 and signal transducer and activator of transcription 3 (STAT3), as well as that for PMP22. These results indicate that IL-6 can enhance PMP22 production in Schwann cells via a JAK2-dependent pathway by probably activating STAT3 and thus may contribute to myelination. © 2010 Elsevier Ireland Ltd. All rights reserved.

収録刊行物

• Neuroscience Letters

  Neuroscience Letters 472 (2), 104-108, 2010-03-19

  Elsevier

キーワード

• gp130
• Interleukin-6
• JAK2
• Peripheral myelin protein 22
• Schwann cell
• STAT3

詳細情報

• CRID: 1050282810930465408
• NII論文ID: 120001939297
• NII書誌ID: AA00754925
• ISSN: 03043940
• Web Site: http://hdl.handle.net/2297/21587
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles