pfaB products determine the molecular species produced in bacterial polyunsaturated fatty acid biosynthesis

• Orikasa, Yoshitake

• Tanaka, Mika

• Sugihara, Shinji

• Hori, Ryuji

• Nishida, Takanori

• Ueno, Akio

• Morita, Naoki

• Yano, Yutaka

• Yamamoto, Kouhei

• Shibahara, Akira

• Hayashi, Hidenori

• Yamada, Yohko

• Yamada, Akiko

• Yu, Reiko

• Watanabe, Kazuo

• Okuyama, Hidetoshi

Abstract

When pDHA4, a vector carrying all five pfaA-pfaE genes responsible for docosahexaenoic acid (DHA; 22:6) biosynthesis in Moritella marina MP-1, was coexpressed in Escherichia coli with the individual pfaA-pfaD genes for eicosapentaenoic acid (EPA; 20:5) biosynthesis from Shewanella pneumatophori SCRC-2738, both polyunsaturated fatty acids were synthesized only in the recombinant carrying pfaB for EPA synthesis. Escherichia coli coexpressing a deleted construct comprising pfaA, pfaC, pfaD and pfaE for EPA and pfaB for DHA produced EPA and DHA. Both EPA and DHA were detected in bacteria that inherently contained pfa genes for DHA. These results suggest that PfaB is the key enzyme determining the final product in EPA or DHA biosynthesis.

Journal

• FEMS Microbiology Letters

  FEMS Microbiology Letters 295 (2), 170-176, 2009-06

  Blackwell Publishing

Keywords

• docosahexaenoic acid
• eicosapentaenoic acid
• Moritella marina strain MP-1
• polyunsaturated fatty acid
• pfaB gene

Details

• CRID: 1050845763931060736
• NII Article ID: 120002128711
• HANDLE: 2115/43086
• ISSN: 03781097
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles