Immortalization of rat spleen and thymus T cells by human T-cell leukemia virus type I.

DOI 機関リポジトリ PubMed

この論文をさがす

抄録

<p>Co-cultivation of thymus and spleen cells of Fisher and Lewis rats with lethally irradiated MT-2 cells harboring human T-cell leukemia virus type I (HTLV-I) resulted in the establishment of lymphoid cell lines, FIRT-1, FIRS-1, LERT-1, and LERS-1, respectively. Cells of these cell lines had rat T-cell characters as demonstrated by the positive reaction to monoclonal antibodies (MAbs) to rat T cell antigens (Thy 1 and pan T). They lacked surface immunoglobulins and strongly expressed rat interleukin-2 receptor antigen (Tac) and Ia antigen. Karyotypic analysis revealed that they had the normal rat karyotype in early cultures, but showed marked aneuploidy after long cultivation. None of them expressed HTLV gag proteins (p19 and p24) or virus particles, but they contained HTLV-I proviral DNA monoclonally and weakly expressed pX gene products (p40x). They were not transplantable into syngeneic newborn rats.</p>

収録刊行物

  • Acta Medica Okayama

    Acta Medica Okayama 43 (3), 143-151, 1989-06

    Okayama University Medical School

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ