Suppression of Cdc27B expression induces plant defence responses

• Kudo, Chikako

• Suzuki, Tomoko

• Fukuoka, Sumie

• Asai, Shuta

• Suenaga, Hiroko

• Sasabe, Michiko

• Takano, Yoshitaka

• Okuno, Tetsuro

• Toyoda, Kazuhiro

• Shiraishi, Tomonori

• Ichinose, Yuki

• Inagaki, Yoshi-Shige

抄録

Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY- 2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase- promoting complex or cyclosome ( APC/ C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus:: Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.

収録刊行物

• Molecular Plant Pathology

  Molecular Plant Pathology 8 (4), 365-373, 2007-07-01

詳細情報

• CRID: 1050002212334873600
• NII論文ID: 120002305195
• Web Site: https://ousar.lib.okayama-u.ac.jp/30136
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles
  • KAKEN