Biochemical study of testosterone in skin: in vivo and in vitro metabolism of testosterone in rat skin

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Abstract

<p>In vitro and in vivo testosterone metabolism was studiedusing rat skin. The in vitro stoichiometric studies strongly suggestedthe existence in the skin of 5a-reductase and other enzymes involvedin testosterone metabolism. The predominant metabolites were 5adihydrotestosterone,androstandiol and 6.4-androstenedione. The rateof testosterone metabolism in vitro was higher in preputial skin than indorsal skin. The main metabolites were androstandiol and 6 4-androstenedionein vivo whereas dihydrotestosterone and androstandiol werepredominant in vitro. .6.4-Pathway is considered to be more active invivo in adults than in newborns. The difference in the in vivo uptakeincrease of testosterone-3H after castration suggests the possible existencein the organs and skin areas of androgen pools of different sizes.The in vivo uptake and metabolism of testosterone were examined inthe nuclei of newborn rats. Dihydrotestosterone and androstandioldetected in the nuclei of preputial skin, dorsal skin and liver were42.8%, 24.3% and 27.8%, of total radioactivity incorporated into therespective organs. The liver incorporated only a small amount ofradioactivity compared with the skin.</p>

Journal

  • Acta Medica Okayama

    Acta Medica Okayama 31(2), 129-139, 1977-04

    Okayama University Medical School

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