Stimulatory effects of insulin-like growth factor 1 on expression of gonadotropin subunit genes and release of follicle-stimulating hormone and luteinizing hormone in masu salmon pituitary cells early in gametogenesis

• Furukuma, Shunji

• Onuma, Takeshi

• Swanson, Penny

• Luo, Qiong

• Koide, Nobuhisa

• Okada, Houji

• Urano, Akihisa

• Ando, Hironori

Bibliographic Information

Other Title

• Stimulatory effects of insulin-like growth factor 1 on expression of gonadotropin subunit genes and release of follicle-stimulating hormone and luteinizing hormone in masu samon pituitary cells early in gametogenesis
• Stimulatory effects of insulin-like growth factor 1 on expression of gonadotropin subunit genes and release of follicle-stimulating hormone and luteinizing hormone in masu salmon pituitary ceft early in gametogenesis

Abstract

Insulin-like growth factor-I (IGF-I) has been shown to be involved in pubertal activation of gonadotropin (GTH) secretion. The aim of this study was to determine if IGF-I directly stimulates synthesis and release of GTH at an early stage of gametogenesis. The effects of IGF-I on expression of genes encoding glycoprotein α (GPα), follicle-stimulating hormone (FSH) β, and luteinizing hormone (LH) β subunits and release of FSH and LH were examined using primary pituitary cells of masu salmon at three reproductive stages: early gametogenesis, maturing stage, and spawning. IGF-I alone or IGF-I + salmon GnRH (sGnRH) were added to the primary pituitary cell cultures. Amounts of GPα, FSHβ, and LHβ mRNAs were determined by real-time PCR. Plasma and medium levels of FSH and LH were determined by RIA. In males, IGF-I increased the amounts of all three subunit mRNAs early in gametogenesis in a dose-dependent manner, but not in the later stages. In females, IGF-I stimulated release of FSH and LH early in gametogenesis, whereas no stimulatory effects on the subunit mRNA levels were observed at any stage. IGF-I + sGnRH stimulated release of FSH and LH at all stages in both sexes, but had different effects on the subunit mRNA levels depending on subunit and stage. The present results suggest that IGF-I itself directly stimulates synthesis and release of GTH early in gametogenesis in masu salmon, possibly acting as a metabolic signal that triggers the onset of puberty.

Journal

• Zoological Science

  Zoological Science 25 (1), 88-98, 2008-01

  Zoological Society of Japan

Keywords

• insulin-like growth factor 1
• gonadotropin-releasing hormone
• gonadotropin
• pituitary
• puberty
• salmon

Details

• CRID: 1050282813982435712
• NII Article ID: 120002414862
• NII Book ID: AA10545874
• DOI: 10.2108/zsj.25.88
• HANDLE: 2115/43960
• NDL BIB ID: 9379390
• ISSN: 02890003
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9379390
• Web Site: http://www.bioone.org/doi/pdf/10.2108/zsj.25.88
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles