Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in Lonicera caerulea var. emphyllocalyx

• Miyashita, Tomomi

• Ohashi, Takafumi

• Shibata, Fukashi

• Araki, Hajime

• Hoshino, Yoichiro

Abstract

An endosperm culture of Haskap (Lonicera caerulea var. emphyllocalyx) was established to develop polyploid plants and investigate the regeneration ability of the endosperm. Based on histological analysis of embryo and endosperm development, endosperms at the globular to early torpedo-stages of developing embryos were used to initiate an endosperm culture. Formation of shoot primordia was observed on Murashige and Skoog (MS) medium (1962) containing benzyladenine (BA) and indole-3-butyric acid (IBA). Shoot primordium formation was confirmed in some genotypes with regeneration frequencies ranging between 1.9% to 10.0%. These proliferated on 1/2 MS medium containing 2.89 μM gibberellic acid (GA3), and then elongated and rooted on MS medium containing 0.44 μM BA and 2.89 μM GA3. These shoots developed into plantlets on 1/2 MS medium. Plantlets maintained ploidy of the endosperm following flow cytometric analysis, thus confirming that these were derived from the endosperm. These results indicated that endosperms were capable of regeneration.

Journal

• Plant Cell, Tissue and Organ Culture

  Plant Cell, Tissue and Organ Culture 98 (3), 291-301, 2009-09

  Springer Netherlands

Keywords

• Aneuploid
• Endosperm culture
• Hexaploid
• Lonicera caerulea
• Ploidy level
• Regeneration

Details

• CRID: 1050564288954450432
• NII Article ID: 120002564616
• NII Book ID: AA10472996
• ISSN: 15735044
• ISSN: 01676857
• HANDLE: 2115/44168
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles