Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa from a natural tetraploid male

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Abstract

Tetraploid loaches have been discovered amongst specimens recovered from fish markets in Japan. These tetraploids can be used as a source of diploid gametes to assist the further expansion of ploidy manipulation. Here, we produced a first generation neo-tetraploid strain by fertilizing eggs from a normal diploid female with diploid spermatozoa from a natural tetraploid male, followed five minutes later by heat shock (42°C, 2 min duration) to inhibit release of the second polar body. Diploid spermatozoa from the neo-tetraploid males produced were then used to create androgenetic diploid progeny by fertilizing UV-irradiated eggs from a normal diploid female. Triploid progeny were produced by crossing a normal diploid female with a neo-tetraploid male. Tetraploid progeny were produced by cold shock (1°C, 40 min duration), beginning 5 min after fertilizing normal eggs with diploid spermatozoa from first generation neo-tetraploid males. Reproductive performance of second generation progeny was also examined. Androgenetic diploid males generated fertile haploid spermatozoa. Triploid males were sterile, but a triploid female laid fertile haploid eggs. Second generation neo-tetraploid males were considered sterile.

Journal

  • Aquaculture

    Aquaculture (308), S133-S139, 2010

    Elsevier

Cited by:  1

Codes

  • NII Article ID (NAID)
    120002564621
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0044-8486
  • Data Source
    CJPref  IR 
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