Effects of Zn[2+] chelators, DTPA and TPEN, and ZnCl2 on the cells treated with hydrogen peroxide: a flow-cytometric study using rat thymocytes

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Recently, we have revealed that trace Zn2+ partly attenuates Ca2+-dependent cell death induced by A23187, a calcium ionophore, in rat thymocytes. In this study, to see if Zn2+ attenuates the H2O2-induced cell death that is also Ca2+-dependent, the effects of ZnCl2 and chelators for Zn2+ have been examined by using a flowcytometer with propidium iodide. The incubation with H2O2 increased the cell lethality. Simultaneousapplication of ZnCl2 greatly augmented the H2O2-induced increase in lethality. DTPA, a chelator for extracellular Zn2+, did not affect the increase in cell lethality by H2O2. However, the H2O2-induced increase in cell lethality was greatly attenuated by TPEN, a chelator for extracellular and intracellular Zn2+. Taken together, it may be likely that intracellular Zn2+ modifies the H2O2-induced cytotoxicity. However, it cannot be ruled out the possibility that TPEN chelates intracellular Fe2+, resulting in inhibiting the formation of hydroxyl radicalfrom H2O2 that leads to an attenuation of H2O2 cytotoxicity.

収録刊行物

  • 自然科学研究

    自然科学研究 23, 13-19, 2009

    徳島大学総合科学部

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各種コード

  • NII論文ID(NAID)
    120002693581
  • NII書誌ID(NCID)
    AN10065859
  • 本文言語コード
    ENG
  • 資料種別
    Departmental Bulletin Paper
  • 雑誌種別
    大学紀要
  • ISSN
    09146385
  • NDL 記事登録ID
    10783979
  • NDL 雑誌分類
    ZM1(科学技術--科学技術一般)
  • NDL 請求記号
    Z14-1395
  • データ提供元
    CJP引用  NDL  IR 
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