Enhanced Intracellular Delivery Using Arginine-Rich Peptides by the Addition of Penetration Accelerating Sequences (Pas)

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Abstract

Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).

Journal

  • Journal of Controlled Release

    Journal of Controlled Release 138(2), 128-133, 2009-09-01

    Elsevier Inc

Cited by:  2

Codes

  • NII Article ID (NAID)
    120002723158
  • NII NACSIS-CAT ID (NCID)
    AA10458678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0168-3659
  • Data Source
    CJPref  IR 
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