Enhanced Intracellular Delivery Using Arginine-Rich Peptides by the Addition of Penetration Accelerating Sequences (Pas)

• Takayama, Kentaro

• Nakase, Ikuhiko

• Michiue, Hiroyuki

• Takeuchi, Toshihide

• Tomizawa, Kazuhito

• Matsui, Hideki

• Futaki, Shiroh

Abstract

Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).

Journal

• Journal of Controlled Release

  Journal of Controlled Release 138 (2), 128-133, 2009-09-01

  Elsevier Inc

Keywords

• arginine-rich peptide
• cell penetrating peptide
• intracellular delivery
• p53 C-terminal segment
• retro-inverso peptide

Details

• CRID: 1050282677155027968
• NII Article ID: 120002723158
• NII Book ID: AA10458678
• ISSN: 01683659
• HANDLE: 2433/134604
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN