Study on the Sliding Friction of Endothelial Cells Cultured on Hydrogel and the Role of Glycocalyx on Friction Reduction

• Chen, Yong Mei

• Kurokawa, Takayuki

• Tominaga, Taiki

• Yasuda, Kazunori

• Osada, Yoshihito

• Gong, Jian Ping

• Yamamoto, Kimiko

• Ando, Joji

Abstract

In this study, we investigated the sliding friction of human umbilical vein endothelial cell (HUVEC) monolayer cultured on poly(sodium p-styrene sulfonate) (PNaSS) gel, intending to elucidate the role of the glycocalyx on the surface of endothelial cell (EC) in friction reduction. Three sets of HUVEC monolayers were investigated: 1) as-cultured HUVEC monolayer, 2) HUVEC monolayer treated by transforming growth factor υ1 (TGF-υ1), which increased glycocalyx by 148%, 3) HUVEC monolayer treated by heparinase I, which reduced glycocalyx by 57%, both were compared with that of the as prepared one. When being slid on flat glass surface, the frictional stress of HUVEC monolayer decreased in the order of heparinase I-treated > as-cultured > TGF-υ1-treated samples. The results suggested that glycocalyx may play a role in reducing the friction of endothelial cell monolayer.

Journal

• Advanced Engineering Materials

  Advanced Engineering Materials 12 (11), B628-B636, 2010-11-17

  WILEY

Keywords

• Friction
• Endothelial cell
• Glycocalyx
• Gel scaffold
• Lubrication

Details

• CRID: 1050282813983891968
• NII Article ID: 120002741677
• NII Book ID: AA11613550
• ISSN: 15272648
• ISSN: 14381656
• HANDLE: 2115/44824
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles