Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41.

• 大石, 真也

• 児玉, 栄一

• Sakakibara, Ayako

• Fukudome, Ayako

• Izumi, Kazuki

• Oishi, Shinya

• Fujii, Nobutaka

• Matsuoka, Masao

抄録

Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents that block HIV-1 fusion, two screening methods based on detection and quantification by the enzyme-linked immunosorbent assay (ELISA) principle have been established. One method uses an alkaline phosphatase (ALP)-conjugated antibody (Ab-ELISA) and the other uses an ALP-fused HR (F-ELISA) to detect and quantify the interaction of the two HRs. The F-ELISA was more simple and rapid, since no ALP-conjugated antibody reaction was required. Both ELISAs detected all the fusion inhibitors tested except for T-20. Interaction of the two HRs was observed in both ELISAs, even in the presence of 10% dimethyl sulfoxide. Ab-ELISA performed best in a pH ranging from 6 to 8, while F-ELISA performed best at a pH ranging from 7 to 8. These results indicate that both established ELISAs are suitable for the identification of HIV-1 fusion inhibitors.

収録刊行物

• Antiviral research

  Antiviral research 80 (1), 71-76, 2008-10

  Elsevier BV

キーワード

• HIV-1
• Gp41
• Fusion
• ELISA
• Screening
• Alkaline phosphatase
• Alkaline Phosphatase/chemistry
• Amino Acid Sequence
• Drug Evaluation, Preclinical/methods
• Enzyme-Linked Immunosorbent Assay/methods
• HIV Envelope Protein gp41/chemistry
• HIV Envelope Protein gp41/metabolism
• HIV Fusion Inhibitors/chemical synthesis
• HIV Fusion Inhibitors/chemistry
• HIV Fusion Inhibitors/pharmacology
• HIV-1/drug effects
• HIV-1/pathogenicity
• Humans
• Membrane Fusion/drug effects
• Repetitive Sequences, Amino Acid/genetics
• Virion/chemistry

詳細情報

• CRID: 1050001202178454784
• NII論文ID: 120002790066
• NII書誌ID: AA10623661
• ISSN: 01663542
• HANDLE: 2433/137211
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles