Spontaneous network activity visualized by ultra-sensitive Ca2+ indicators, yellow Cameleon-Nano

• Horikawa, Kazuki

• Yamada, Yoshiyuki

• Matsuda, Tomoki

• Kobayashi, Kentarou

• Hashimoto, Mitsuhiro

• Matsu-ura, Toru

• Miyawaki, Atsushi

• Michikawa, Takayuki

• Mikoshiba, Katsuhiko

• Nagai, Takeharu

Abstract

We report a new set of ultra-sensitive Ca2+ indicators, yellow cameleon-Nano (YC-Nano), developed by engineering the Ca2+-sensing domain of a genetically encoded Ca2+ indicator, YC2.60 or YC3.60. Their high Ca2+ affinities (Kd = 15-140 nM) and large signal change (1,450%) enabled detection of subtle Ca2+ transients associated with intercellular signaling dynamics and neuronal activity, even in 100,000-cell networks. These indicators will be useful for studying information processing in living multi-cellular networks.

Journal

• Nature Methods

  Nature Methods 7 (9), 729-732, 2010-09

  Nature Publishing Group

Details

• CRID: 1050845763937105664
• NII Article ID: 120002834691
• HANDLE: 2115/44903
• ISSN: 15487091
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles