Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation

• Fujimori, Chika

• Ogiwara, Katsueki

• Hagiwara, Akane

• Rajapakse, Sanath

• Kimura, Atsushi

• Takahashi, Takayuki

抄録

In vitro ovulation of mature medaka ovarian follicles was inhibited by inhibitors of cyclooxygenase (COX) or by an antagonist of the prostaglandin E2 receptor (EP). Of the three medaka COX genes, ptgs2 was most dominantly expressed in the fish ovary. The ptgs2 transcript was detected in all sizes of growing follicles. In a 24-h spawning cycle, large-sized follicles contained ptgs2 mRNA at a fairly constant level. The levels of COX enzyme activity and prostaglandin E2 were also constant in the large-sized follicles during the spawning cycle. The expression of prostaglandin E2 receptor EP4b (ptger4b) mRNA was drastically upregulated in the large-sized follicles as the ovulation time approached. The current results indicate that prostaglandin E2. which might be produced by COX-2, is involved in the ovulation of medaka, and that EP4b is likely the receptor responsible for exerting the action of prostaglandin E2 in the process.

収録刊行物

• Molecular and Cellular Endocrinology

  Molecular and Cellular Endocrinology 332 (1-2), 67-77, 2011-01-30

  Elsevier Ireland

キーワード

• Prostaglandin E2
• Cyclooxygenase-2
• EP receptor
• Gene expression
• Ovulation
• Medaka

詳細情報

• CRID: 1050282813983944448
• NII論文ID: 120002834743
• HANDLE: 2115/45012
• ISSN: 03037207
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles