The effect of Ndrg2 expression on astroglial activation

• Takeichi, Toshiaki

• Takarada-Iemata, Mika

• Hashida, Koji

• Sudo, Hirofumi

• Okuda, Tomohiko

• Kokame, Koichi

• Hatano, Taku

• Takanashi, Masashi

• Funabe, Sayaka

• Hattori, Nobutaka

• Kitamura, Osamu

• Kitao, Yasuko

• Hori, Osamu

抄録

N-myc downstream-regulated gene 2 (Ndrg2) is a differentiation- and stress-associated molecule predominantly expressed in astrocytes in the central nervous system (CNS). To study the expression and possible role of Ndrg2 in quiescent and activated astrocytes, mice were administrated with 1-methyl-4-phenyl-1,2,3,6-tetrahydropypridine (MPTP), a Parkinson disease (PD)-related neurotoxin which causes both neurodegeneration and glial activation. Immunohistological analysis revealed that Ndrg2 was highly expressed in both types of astrocytes, but less so in astrocytes during the early process of activation. Ndrg2 was also expressed in astrocyte-like cells, but not in neurons, in human brains from PD and Cortico-basal degeneration (CBD) patients. In cultured astrocytes, gene silencing of Ndrg2 significantly enhanced the numbers of 5-bromo-2′-deoxy-uridine (BrdU)-incorporated and proliferating cell nuclear antigen (PCNA)-positive cells, and reduced the length of cell processes and the amount of F-actin. In contrast, adenovirus-mediated overexpression of Ndrg2 significantly reduced the numbers of BrdU-incorporated and PCNA-positive cells, and enhanced the amount of F-actin. Fractionation and immunocytochemical analysis further revealed that Ndrg2 was located in different cellular fractions including the cytosol and cell surface membranes. These results suggest that Ndrg2 may regulate astroglial activation through the suppression of cell proliferation and stabilization of cell morphology. © 2011 Elsevier Ltd. All rights reserved.

収録刊行物

• Neurochemistry International

  Neurochemistry International 59 (1), 21-27, 2011-08-01

  Elsevier

キーワード

• Cell morphology
• Cell proliferation
• Neurodegenerative diseases
• Reactive astrocytosis

詳細情報

• CRID: 1050001335953771776
• NII論文ID: 120003221535
• NII書誌ID: AA0032399X
• ISSN: 01970186
• Web Site: http://hdl.handle.net/2297/28496
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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