Solubilization of the ecdysone binding protein from anterior silk gland cell membranes of the silkworm, bombyx mori

• Elmogy, Mohamed

• Iwami, Masafumi

• Sakurai, Sho

抄録

We previously provided preliminary evidence for the presence of a putative membrane ecdysone receptor (mEcR) anchored in the plasma membranes of anterior silk glands (ASGs) in Bombyx mori. This receptor may act in concert with the conventional EcR in 20E-dependent programmed cell death of these glands. We report here, for the first time, the solubilization of mEcR from ASG membranes using the zwitterionic detergent CHAPS in the presence of NaCl. Our results show by ligand binding assay that mEcR solubilized this way is functionally active and retains 75% of its native binding activity. We also defined experimental conditions that yielded protein/detergent complexes with partial binding activity, which makes it possible to purify the membrane-bound ecdysone binding protein. © 2007 Zoological Society of Japan.

収録刊行物

• Zoological science

  Zoological science 24 (10), 971-977, 2007-10-01

  Zoological Society of Japan = 日本動物学会

キーワード

• Anterior silk gland
• Bombyx mori
• Detergent
• Ecdysteorid
• Membrane ecdysone receptor
• Solubilization

詳細情報

• CRID: 1050282810919217024
• NII論文ID: 120003291017
• NII書誌ID: AA10545874
• ISSN: 02891824
• ISSN: 02890003
• DOI: 10.2108/zsj.24.971
• NDL書誌ID: 9749003
• Web Site: http://hdl.handle.net/2297/28990
• Web Site: http://id.ndl.go.jp/bib/9749003
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9749003
• Web Site: http://www.bioone.org/doi/pdf/10.2108/zsj.24.971
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
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  • NDL
  • Crossref
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