Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli

• Kitamatsu, Mizuki

• Kurami, Shunsuke

• Ohtsuki, Takashi

• Sisido, Masahiko

Abstract

To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures.

Journal

• Bioorganic & Medicinal Chemistry Letters

  Bioorganic & Medicinal Chemistry Letters 21 (1), 225-227, 2011-01

  Pergamon-Elsevier Science Ltd,

Keywords

• Peptide nucleic acid
• Cell-penetrating peptide
• Antisense effect

Details

• CRID: 1050283687312405504
• NII Article ID: 120003353368
• NII Book ID: AA1079577X
• ISSN: 0960894X
• Web Site: https://ousar.lib.okayama-u.ac.jp/46919
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles