Facile conversion of RNA aptamers to modular fluorescent sensors with tunable detection wavelengths.

• Nakano, Shun

• Nakata, Eiji

• Morii, Takashi

Abstract

A GTP aptamer was converted to a modular fluorescent GTP sensor by conjugation of RRE (Rev responsive element) RNA and successive complex formation with a fluorophore-modified Rev peptide. Structural changes associated with substrate binding in the RNA aptamer were successfully transduced into changes in fluorescence intensity because of the modular structure of ribonucleopeptides. A simple modular strategy involving conjugation of a fluorophore-modified ribonucleopeptide to the stem region of an RNA aptamer deduced from secondary structural information helps produce fluorescent sensors, which allow tuning of excitation and detection wavelengths through the replacement of the fluorophore at the N-terminal of the Rev peptide.

Journal

• Bioorganic & medicinal chemistry letters

  Bioorganic & medicinal chemistry letters 21 (15), 4503-4506, 2011-08-01

  Elsevier Ltd.

Keywords

• Aptamer
• Ribonucleopeptide
• Biosensor
• Modular design
• Fluorescence detection

Details

• CRID: 1050845760651068032
• NII Article ID: 120003367878
• NII Book ID: AA11522706
• ISSN: 0960894X
• DOI: 10.1016/j.bmcl.2011.05.120
• HANDLE: 2433/147154
• Web Site: https://api.elsevier.com/content/article/PII:S0960894X1100761X?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0960894X1100761X?httpAccept=text/plain
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN