Viral-mediated stabilization of AU-rich element containing mRNA contributes to cell transformation

• Kuroshima, Takeshi

• Aoyagi, Mariko

• Yasuda, Motoaki

• Kitamura, Tetsuya

• Jehung, Jumond P.

• Ishikawa, Makoto

• Kitagawa, Yoshimasa

• Totsuka, Yasunori

• Shindoh, Masanobu

• Higashino, Fumihiro

Abstract

E4orf6 is one of the oncogene products of adenovirus and it also plays an important role for transportation of cellular and viral mRNA during the late phase of virus infection. We previously revealed that E4orf6 controls the fate of AU-rich element (ARE) containing mRNA by perturbing the CRM1-dependent export mechanism. Here, we show that E4orf6 stabilizes ARE-mRNA through the region required for its oncogenic activity and ubiquitin E3 ligase assembly. Cells that failed to stabilize ARE-mRNA after HuR knockdown were unable to produce colonies in soft-agar, even when E4orf6 was expressed. Furthermore, the stabilized ARE-mRNA induced the transformation of rodent immortalized cells. These findings indicate that stabilized ARE-mRNA is necessary, if not all, for the oncogenic activity of E4orf6 and has the potential to transform cells at least under a certain condition.

Journal

• Oncogene

  Oncogene 30 (26), 2912-2920, 2011-06-30

  Nature Publishing Group

Keywords

• adenovirus
• AU-rich element
• E4orf6
• transformation
• stabilization

Details

• CRID: 1050564288961822208
• NII Article ID: 120003660587
• HANDLE: 2115/47941
• ISSN: 09509232
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles