Cure of ADPKD by Selection for Spontaneous Genetic Repair Events in Pkd1-Mutated iPS Cells

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Abstract

マウスiPS細胞を用いた遺伝性疾患の治療モデル-多発性嚢胞腎(ADPKD)モデルマウスの治療-. 京都大学プレスリリース. 2012-2-10.Induced pluripotent stem cells (iPSCs) generated by epigenetic reprogramming of personal somatic cells have limited therapeutic capacity for patients suffering from genetic disorders. Here we demonstrate restoration of a genomic mutation heterozygous for Pkd1 (polycystic kidney disease 1) deletion (Pkd1(+/−) to Pkd1(+/R+)) by spontaneous mitotic recombination. Notably, recombination between homologous chromosomes occurred at a frequency of 1~2 per 10,000 iPSCs. Southern blot hybridization and genomic PCR analyses demonstrated that the genotype of the mutation-restored iPSCs was indistinguishable from that of the wild-type cells. Importantly, the frequency of cyst generation in kidneys of adult chimeric mice containing Pkd1(+/R+) iPSCs was significantly lower than that of adult chimeric mice with parental Pkd1(+/−) iPSCs, and indistinguishable from that of wild-type mice. This repair step could be directly incorporated into iPSC development programmes prior to cell transplantation, offering an invaluable step forward for patients carrying a wide range of genetic disorders.

Journal

  • PLoS ONE

    PLoS ONE 7(2), e32018, 2012-02-09

    Public Library of Science

Cited by:  1

Codes

  • NII Article ID (NAID)
    120003796491
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    1932-6203
  • Data Source
    CJPref  IR 
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