PET imaging of hypoxia-inducible factor-1-active tumor cells with pretargeted oxygen-dependent degradable streptavidin and a novel [18]F-labeled biotin derivative.
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<Purpose> : We aimed to evaluate the feasibility of using streptavidin–biotin-based pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor (HIF)-1-active tumors. <Procedures> : We used POS, a genetically engineered form of streptavidin that selectively stabilizes in HIF-1-active cells, and (4-[18]F-fluorobenzoyl)norbiotinamide ([18]F-FBB), a radiolabeled biotin derivative, for performing a biodistribution study and for PET imaging. The tumoral [18]F-FBB accumulation was compared to the HIF-1-dependent luciferase bioluminescence and HIF-1α immunohistochemical signal. <Results> : [18]F-FBB accumulation was observed in POS-pretargeted tumors in mice (2.85 ± 0.55% injected dose per gram at 3 h), and clear PET images were obtained at the same time point. The tumoral [18]F-FBB accumulation positively correlated with luciferase bioluminescence (R = 0.72, P < 0.05), and most of the area showing [18]F-FBB accumulation corresponded to HIF-1α-positive areas. <Conclusion> : Pretargeting with POS and [18]F-FBB is an effective approach for PET imaging of HIF-1-active areas in tumors.
収録刊行物
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- Molecular imaging and biology
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Molecular imaging and biology 13 (5), 1003-1010, 2011-10
Springer
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キーワード
- Tumor hypoxia
- Hypoxia-inducible factor-1 (HIF-1)
- Oxygen-dependent degradation domain (ODD)
- Pretargeting
- [18]F-labeled biotin derivative
- Animals
- Biotin/metabolism
- Cell Line, Tumor
- Fluorine Radioisotopes/metabolism
- Hypoxia-Inducible Factor 1/metabolism
- Immunohistochemistry
- Mice
- Oxygen/metabolism
- Positron-Emission Tomography
- Streptavidin
詳細情報 詳細情報について
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- CRID
- 1050564285679815808
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- NII論文ID
- 120003824938
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- NII書誌ID
- AA1170000X
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- ISSN
- 15361632
- 18602002
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- HANDLE
- 2433/153020
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- 本文言語コード
- en
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- 資料種別
- journal article
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- データソース種別
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- IRDB
- CiNii Articles