Genotyping of sand fly species in Peruvian Andes where leishmaniasis is endemic

• Fujita, Megumi

• Kato, Hirotomo

• Cáceres, Abraham G.

• Gomez, Eduardo A.

• Velez, Lenin

• Mimori, Tatsuyuki

• Zhang, Feifei

• Iwata, Hiroyuki

• Korenaga, Masataka

• Sakurai, Tatsuya

• Katakura, Ken

• Hashiguchi, Yoshihisa

Abstract

Genotyping of sand fly species circulating in Peru was established on the basis of PCR-restriction fragment length polymorphisms (RFLPs) of the 18S ribosomal RNA (rRNA) gene. The sequences of 18S rRNA gene fragments from 12 Lutzomyia and 1 Warileya species were determined and their RFLP-patterns were analyzed. Consequently, RFLP analysis with the restriction enzyme AfaI and then HapII or KpnI, followed by XspI successfully differentiated them. Intraspecific genetic diversity affecting RFLP-patterns was not detected in the specimens collected from 24 areas of 8 departments. The genotyping was applied to the surveillance of sand flies collected from Andean areas where leishmaniasis is endemic, and its usability was verified. The present method promises to be a powerful tool for the classification and surveillance of sand flies circulating in Peru.

Journal

• Acta Tropica

  Acta Tropica 121 (2), 93-98, 2012-02

  Elsevier B.V.

Keywords

• Genotyping
• Sand fly
• Lutzomyia
• PCR-RFLP
• 18S rRNA gene

Details

• CRID: 1050001202676432640
• NII Article ID: 120003860279
• HANDLE: 2115/48545
• ISSN: 0001706X
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN