Atg14, a key player in orchestrating autophagy

• Obara, Keisuke

• Ohsumi, Yoshinori

Abstract

Phosphorylation of phosphatidylinositol (PtdIns) by a PtdIns 3-kinase is an essential process in autophagy. Atg14, a specific subunit of one of the PtdIns 3-kinase complexes, targets the complex to the probable site of autophagosome formation, thereby, sorting the complex to function specifically in autophagy. The N-terminal half of Atg14, containing coiled-coil domains, is required to form the PtdIns 3-kinase complex and target it to the proper site. The C-terminal half of yeast Atg14 is suggested to be involved in the formation of a normal-sized autophagosome. The C-terminal half of mammalian Atg14 contains the Barkor/Atg14(L) autophagosome-targeting sequence (BATS) domain that preferentially binds to the highly curved membranes containing PtdIns(3)P and is proposed to target the PtdIns 3-kinase complex efficiently to the isolation membrane. Thus, the Nand C-terminal halves of Atg14 are likely to have an essential core function and a regulatory role, respectively.

Journal

• International Journal of Cell Biology

  International Journal of Cell Biology 2011 713435-, 2011-10-13

  Hindawi Publishing

Details

• CRID: 1050845763940697344
• NII Article ID: 120003878522
• ISSN: 16878884
• ISSN: 16878876
• HANDLE: 2115/48556
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles