The Distribution of a Phage-Related Insertion Sequence Element in the Cyanobacterium, Microcystis aeruginosa
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The cyanophage Ma-LMM01, specifically-infecting Microcystis aeruginosa, has an insertion sequence (IS) element that we named IS607-cp showing high nucleotide similarity to a counterpart in the genome of the cyanobacterium Cyanothece sp. We tested 21 strains of M. aeruginosa for the presence of IS607-cp using PCR and detected the element in strains NIES90, NIES112, NIES604, and RM6. Thermal asymmetric interlaced PCR (TAIL-PCR) revealed each of these strains has multiple copies of IS607-cp. Some of the ISs were classified into three types based on their inserted positions; IS607-cp-1 is common in strains NIES90, NIES112 and NIES604, whereas IS607-cp-2 and IS607-cp-3 are specific to strains NIES90 and RM6, respectively. This multiplicity may reflect the replicative transposition of IS607-cp. The sequence of IS607-cp in Ma-LMM01 showed robust affinity to those found in M. aeruginosa and Cyanothece spp. in a phylogenetic tree inferred from counterparts of various bacteria. This suggests the transfer of IS607-cp between the cyanobacterium and its cyanophage. We discuss the potential role of Ma-LMM01-related phages as donors of IS elements that may mediate the transfer of IS607-cp; and thereby partially contribute to the genome plasticity of M. aeruginosa.
収録刊行物
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- Microbes and Environments
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Microbes and Environments 25 (4), 295-301, 2010
Japanese Society of Microbial Ecology
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詳細情報 詳細情報について
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- CRID
- 1571698602549492352
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- NII論文ID
- 120004184961
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- NII書誌ID
- AA11173196
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- ISSN
- 13426311
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- Web Site
- http://hdl.handle.net/2433/156275
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- 本文言語コード
- en
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- データソース種別
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