第13回徳島医学会賞受賞論文 性分化と精子形成機構に対するプロテオミクスからのアプローチ  [in Japanese] Proteomics approach for the mechanism of sex determination  [in Japanese]

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Abstract

SRY (sex determining region on the Y chromosome) was a gene isolated as the testis determining factor on the Y chromosome in 1990.The gene consisted of single exon and encodes a 204 amino acid protein. Since SRY protein has a HMG domain, which functions as DNA binding domain, it has been considered to be a transcription factor. Direct target of SRY, however, have not yet identified for 14 years.In this study, we have performed a proteomics approach to analyze the function of SRY. Twodimensional gel electrophoresis after over expression of SRY showed considerable down regulation in many proteins. Among the proteins, mitochondrial 60‐kDa heat shock protein (HSP60) and probable protein disulfide isomerase (ER60) remarkably decreased. Since they were reported to be associated with cell proliferation and differentiation, we investigated the effects of SRY on cell cycle profiles. It was shown that the over‐expression of SRY negatively regulated the cell growth with significant S or G2/M arrest of cell cycle.

Journal

  • Shikoku acta medica

    Shikoku acta medica 60(5・6), 168-171, 2004-12-20

    徳島医学会

Codes

  • NII Article ID (NAID)
    120004631530
  • NII NACSIS-CAT ID (NCID)
    AN00102041
  • Text Lang
    JPN
  • Article Type
    journal article
  • Journal Type
    大学紀要
  • ISSN
    00373699
  • NDL Article ID
    7284419
  • NDL Source Classification
    ZS7(科学技術--医学)
  • NDL Call No.
    Z19-344
  • Data Source
    NDL  IR 
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