New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes.

• Fujimori, Chika

• Ogiwara, Katsueki

• Hagiwara, Akane

• Takahashi, Takayuki

抄録

A cDNA for a prostaglandin E(2) (PGE(2)) receptor subtype 4, EP4b (Ptger4b), was cloned from the medaka ovary. The effect of PGE(2) was examined using COS-7 cells expressing the recombinant Ptger4b protein. An increase in intracellular cAMP levels was observed when the cells were incubated with PGE(2), but the increase in cAMP levels was nullified by the addition of the EP4 antagonist GW627368X. The expression of ptger4b mRNA was drastically induced by the addition of pregnant mare serum gonadotropin to the in vitro culture of large preovulatory follicles. In in vitro ovulation studies of the effect of GW627368X addition on follicle ovulation, the critical timing of the PGE(2)/Ptger4b interaction was suggested to be between -1 and 0h of ovulation. These results further substantiate that PGE(2)/Ptger4b signaling is involved in follicle rupture during ovulation in the medaka ovary.

収録刊行物

• Molecular and cellular endocrinology

  Molecular and cellular endocrinology 362 (1-2), 76-84, 2012-10-15

  Elsevier Science

キーワード

• Prostaglandin E2
• EP receptor
• Intracellular cAMP level
• Gene expression
• Ovulation
• Medaka

詳細情報

• CRID: 1050564288965314048
• NII論文ID: 120004650807
• HANDLE: 2115/49982
• ISSN: 18728057
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles