Identification and expression analysis of the genes involved in serotonin biosynthesis and transduction in the field cricket Gryllus bimaculatus

• Watanabe, Takayuki

• Sadamoto, Hisayo

• Aonuma, Hitoshi

抄録

Serotonin (5-HT) modulates various aspects of behaviours such as aggressive behaviour and circadian behaviour in the cricket. To elucidate the molecular basis of the cricket 5-HT system, we identified 5-HT-related genes in the field cricket Gryllus bimaculatus DeGeer. Complementary DNA of tryptophan hydroxylase (TRH) and phenylalanine-tryptophan hydroxylase (TPH) which convert tryptophan into 5-hydroxy-L-tryptophan (5-HTP), and that of aromatic L-amino acid decarboxylase (AADC) which converts 5-HTP into 5-HT were isolated from a cricket brain cDNA library. In addition, four 5-HT receptor genes (5-HT_[1A], 5-HT_[1B], 5-HT_[2α], and 5-HT7) were identified. Expression analysis of TRH and TPH, which are selectively involved in neuronal and peripheral 5-HT synthesis in Drosophila, suggested that two 5-HT synthesis pathways co-exist in the cricket neuronal tissues. The four of 5-HT receptor genes were expressed in various tissues at differential expression levels, suggesting that the 5-HT system is widely distributed in the cricket.

収録刊行物

• Insect Molecular Biology

  Insect Molecular Biology 20 (5), 619-635, 2011-10

  Wiley-Blackwell

キーワード

• serotonin
• tryptophan hydroxylase
• phenylalanine-tryptophan hydroxylase
• aromatic L-amino acid decarboxylase
• serotonin receptors
• Gryllus bimaculatus

詳細情報

• CRID: 1050001339009447168
• NII論文ID: 120004710961
• HANDLE: 2115/50147
• ISSN: 09621075
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles