Visualization of mobility by atomic force microscopy
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Intrinsically disordered regions (IDRs) of proteins are very thin and hence hard to be visualized by electron microscopy. Thus far, only high-speed atomic force microscopy (HS-AFM) can visualize them. The molecular movies identify the alignment of IDRs and ordered regions in an intrinsically disordered protein (IDP) and show undulation motion of the IDRs. The visualized tail-like structures contain the information of mechanical properties of the IDRs. Here, we describe methods of HS-AFM visualization of IDPs and methods of analyzing the obtained images to characterize IDRs. © 2012 Springer Science+Business Media New York.
- Methods in Molecular Biology
Methods in Molecular Biology (896), 57-69, 2012-01-01
Springer Science+Business Media