RNA Interference Induction by Long Hairpin dsRNAs Expressed from Chromosomal DNA of Bombyx mori Cells

DOI HANDLE Open Access
  • Lee Jae Man
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Kojin Yoshito
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Tatsuke Tsuneyuki
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Mon Hiroaki
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences
  • Miyagawa Yoshitaka
    Department of Developmental Biology, National Research Institute for Child Health and Development
  • Kusakabe Takahiro
    Laboratory of Silkworm Sciences, Kyushu University Graduate School of Bioresource and Bioenvironmental Sciences

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Abstract

RNA interference (RNAi) triggered by long double–stranded RNA (dsRNA) transcribed from chromosomal DNA has a lot of advantages in high–throughput analyses of gene function. In this report, we have constructed Gateway®–based RNAi induction vectors, by which we efficiently integrated expression cassettes for long hairpin dsRNA into chromosomal DNA of Bombyx mori cells. RNAi induced by the hairpin dsRNAs using our constructs decreased a reporter gene activity by approximately 70–80% in cultured B. mori cells.

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Details 詳細情報について

  • CRID
    1390853649614041088
  • NII Article ID
    120004756198
  • NII Book ID
    AA00247166
  • DOI
    10.5109/25203
  • HANDLE
    2324/25203
  • ISSN
    00236152
  • Text Lang
    en
  • Data Source
    • JaLC
    • IRDB
    • Crossref
    • CiNii Articles

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