The Roles of Angiotensin II in Stretched Periodontal Ligament Cells
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- Monnouchi, Satoshi
- Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
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- Maeda, Hidefumi
- Department of Endodontology, Kyushu University Hospital
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- Tomokiyo, Atsushi
- Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
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- Kono, Kiyomi
- Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
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- Akamine, Akifumi
- Department of Endodontology, Kyushu University Hospital | Division of Oral Rehabilitation, Department of Endodontology and Operative Dentistry, Faculty of Dental Science, Kyushu University
Abstract
The loading caused by occlusion and mastication plays an important role in maintaining periodontal ligament (PDL) tissues. We hypothesized that a loading magnitude would be involved in the production of biological factors that function in the maintenance of PDL tissues. Here, we identified up-regulated gene expressions of transforming growth factor-β1 (TGF-β1), alkaline phosphatase (ALP), and angiotensinogen in human PDL fibroblastic cells (HPLFs) that were exposed to 8% stretch loading. Immunolocalization of angiotensin I/II (Ang I/II), which was converted from angiotensinogen, was detected in rat PDL tissues. HPLFs that were stimulated by Ang II also increased their gene expressions of TGF-β1 and ALP. Furthermore, the antagonist for Ang II type 2 receptor, rather than for type 1, significantly inhibited gene expressions induced by the stretch loading. Analysis of these data suggests that Ang II mediates the loading signal in stretched HPLFs to induce expressions of TGF-β1 and ALP.
Journal
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- Journal of Dental Research
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Journal of Dental Research 90 (2), 181-185, 2011-02
International & American Associations for Dental Research
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Keywords
- stretch
- alkaline phosphatase
- angiotensin II
- angiotensinogen
- osteoclast differentiation factor
- osteoprotegerin
- recombinant protein
- transforming growth factor beta1
- adult
- animal
- article
- cell culture
- cytology
- dental care
- female
- fibroblast
- gene expression
- genetics
- human
- male
- mechanical stress
- metabolism
- periodontal ligament
- physiology
- rat
- upregulation
- Animals
- Cells, Cultured
- Dental Stress Analysis
- Fibroblasts
- Gene Expression
- Humans
- Osteoprotegerin
- Periodontal Ligament
- RANK Ligand
- Rats
- Recombinant Proteins
- Stress, Mechanical
- Up-Regulation
Details 詳細情報について
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- CRID
- 1050580007681406848
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- NII Article ID
- 120004951569
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- HANDLE
- 2324/25451
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- Text Lang
- en
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- Article Type
- journal article
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- Data Source
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- IRDB
- CiNii Articles