Effective DNA extraction method for fragment analysis using capillary sequencer of the kelp, Saccharina

• Maeda, Takashi

• Kawai, Tadashi

• Nakaoka, Masahiro

• Yotsukura, Norishige

Abstract

The DNA fragment analysis can become an effective tool to study genetic differences between not only species but also individuals on saccharinan kelp from which the little genetic diversity was reported. This time, extraction methods of suitable DNA for use in the analysis with a capillary sequencer was examined on Saccharina japonica var. diabolica that contains polysaccharide abundantly. When AFLP was performed using genomic DNA extracted by seven different methods: (1) commercial kit, (2) original CTAB method, (3)-(5) three types of modified CTAB method, (6) modified SDS method, (7) combination of CTAB method and SDS method, high reproducible peak that was worth for the analysis was noticeable in the electropherogram in the experiment with the last combination method (7). It is considered that the pretreatment washing of polysaccharide and the subsequent purification for protein and RNA in SDS method and for polysaccharide in CTAB method are effective to obtain the high purity DNA.

Journal

• Journal of Applied Phycology

  Journal of Applied Phycology 25 (1), 337-347, 2013-02

  Springer Netherlands

Keywords

• AFLP
• DNA extraction
• DNA purification
• Fragment analysis
• Saccharina
• Phaeophyta

Details

• CRID: 1050282813989945984
• NII Article ID: 120005228165
• DOI: 10.1007/s10811-012-9868-3
• ISSN: 15735176
• ISSN: 09218971
• HANDLE: 2115/52032
• Web Site: http://link.springer.com/content/pdf/10.1007/s10811-012-9868-3.pdf
• Web Site: http://link.springer.com/article/10.1007/s10811-012-9868-3/fulltext.html
• Web Site: http://link.springer.com/content/pdf/10.1007/s10811-012-9868-3
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • Crossref
  • CiNii Articles
  • KAKEN