Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain

• Ishihara, Seiichiro

• Yasuda, Motoaki

• Nishioka, Takeshi

• Mizutani, Takeomi

• Kawabata, Kazushige

• Shirato, Hiroki

• Haga, Hisashi

Abstract

Radiotherapy is one of the major treatment modalities for malignancies. However, cells surviving irradiation often display high levels of invasiveness. This study shows that irradiation-tolerant lung adenocarcinoma demonstrates high invasive capability depending on dephosphorylation of the myosin regulatory light chain (MRLC). In a collagen gel overlay condition, low-invasive subclones of lung adenocarcinoma (A549P-3) showed a round morphology and diphosphorylation of MRLC. In contrast, irradiation-tolerant A549P-3 cells (A549P-3IR) displayed high invasiveness and a lower level of MRLC diphosphorylation. In addition, inhibition of MRLC phosphatase activity decreased the invasive activity. These findings suggest that A549P-3IR cells acquire high invasiveness through MRLC dephosphorylation. (C) 2013 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

Journal

• FEBS Letters

  FEBS Letters 587 (6), 732-736, 2013-03-18

  Elsevier

Keywords

• 3D culture
• Collagen gel
• Irradiation
• Invasion
• Lung adenocarcinoma
• Myosin regulatory light chain

Details

• CRID: 1050001339013399168
• NII Article ID: 120005228631
• HANDLE: 2115/52653
• ISSN: 00145793
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles