Skeletal muscle necrosis in rat induced by Trimeresurus flavoviridis venom can be prevented by its serum proteins
Trimeresurus flavoviridis (Tf) serum proteins were fractionated by anmnonium sulfate precipitation to five portions depending on the differences of its saturation percentages, that is, 0-20%, 20-30%, 30-40%, 40-50%, and 50-70%. The effects of these proteins on Tf venom-induced rat skeletal muscle damage were investigated with closer attention to histopathological features of impairment, necrosis, and regeneration ofmuscle fibers. The knowledges which portion of Tf serum proteins is effective for prevention of local lesions caused by Tf venom should shed light on the effective medical treatment after bitten by Tf snake. In consequence, it was found that the necrotic change of the rats inoculated with Tf crude venom together with the serum protein fraction of ammonium sulfate saturation percentage 40-50% was the smallest compared to those of the rats tested with other Tf serum protein fractions.
南太平洋研究 23(1), 11-18, 2002