A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae.
Bibliographic Information
- Other Title
-
- A fluorescence resonance energy transfer (FRET)‐based redox sensor reveals physiological role of thioredoxin in the yeast <i>Saccharomyces cerevisiae</i>
Search this article
Abstract
The physiological roles of the thioredoxin isozymes in the yeast Saccharomyces cerevisiae were investigated using a novel FRET-based redox probe, Redoxfluor. After establishing responsiveness of the probe toward thioredoxin, we followed the fluorescence signal of Redoxfluor expressed in the yeast and found that one of the thioredoxin isozymes, Trx2, was required for maintaining the redox status when stationary culture of the organism was exposed to starvation and mild-heat stresses. The failure to maintain redox balance under the tested condition preceded decreased viability of the trx2 mutants, indicating the functional importance of the cytoplasmic thioredoxin in adaptation to environmental changes.
Journal
-
- FEBS letters
-
FEBS letters 587 (6), 793-798, 2013-03-18
Elsevier B.V.
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1050564285720726656
-
- NII Article ID
- 120005244260
-
- NII Book ID
- AA00642943
-
- ISSN
- 00145793
- 18733468
-
- HANDLE
- 2433/173130
-
- Text Lang
- en
-
- Article Type
- journal article
-
- Data Source
-
- IRDB
- Crossref
- CiNii Articles
- KAKEN