Sequence-specific electron injection into DNA from an intermolecular electron donor.

• Morinaga, Hironobu

• Takenaka, Tomohiro

• Hashiya, Fumitaka

• Kizaki, Seiichiro

• Hashiya, Kaori

• Bando, Toshikazu

• Sugiyama, Hiroshi

Abstract

Electron transfer in DNA has been intensively studied to elucidate its biological roles and for applications in bottom-up DNA nanotechnology. Recently, mechanisms of electron transfer to DNA have been investigated; however, most of the systems designed are intramolecular. Here, we synthesized pyrene-conjugated pyrrole-imidazole polyamides (PPIs) to achieve sequence-specific electron injection into DNA in an intermolecular fashion. Electron injection from PPIs into DNA was detected using 5-bromouracil as an electron acceptor. Twelve different 5-bromouracil-containing oligomers were synthesized to examine the electron-injection ability of PPI. Product analysis demonstrated that the electron transfer from PPIs was localized in a range of 8 bp from the binding site of the PPIs. These results demonstrate that PPIs can be a useful tool for sequence-specific electron injection.

Journal

• Nucleic acids research

  Nucleic acids research 41 (8), 4724-4728, 2013-04-01

  Oxford University Press.

Details

• CRID: 1050845760699972608
• NII Article ID: 120005245128
• NII Book ID: AA00760269
• ISSN: 03051048
• HANDLE: 2433/174316
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles