ヘッドスペース法を用いた血中一酸化炭素濃度測定の有効性 (山本淳教授 退職記念号) [in Japanese] Validity for Measuring Blood CO Concentration by Head Space Method [in Japanese]
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Carbon monoxide （CO） concentration in the blood is measured by a headspacemethod with a gas-chromatography （HS-GC） and/or a CO-oximeter. However, thedeficiencies of using HS-GC, especially on blood CO analysis, are not welldocumented. In the present study the effect of these deficiencies and the agreementof the results between the HS-GC and CO-oximeter methods were examined. Resultsindicated, in the application of HS-GC for blood CO analysis, that the TRIlyzer, asimplified gas chromatograph used in this study, showed a high linearity andreproducibility. The use of a surfactant such as Triton enhanced destruction of theplasma membrane of the erythrocytes and thereby increased blood CO values.Furthermore, an alkaline buffer solution provided higher blood CO values comparedwith those of an acid buffer solution. Correlation analysis between the results obtainedby HS-GC and CO-oximeter on four subjects, including smokers and non-smokers,also revealed a significant relationship （r= 0.954） between them. However, thecorrelation coefficient （r） between CO concentrations during and after exercisemeasured by HS-GC and CO-oximeter on each subject showed a negative correlationfor three of the four subjects. It is suggested that, in the application of HS-GC, asurfactant and an alkaline buffer solution should be used in the blood CO analysis, butdiscrepancies still remain between the values obtained by HS-GC and CO-oximeter.Therefore, further studies will be required to obtain clear agreement between themethods.
- 雲雀野 : 豊橋技術科学大学人文科学系紀要 = The lark hill : bulletin of the School of Humanities, Toyohashi University of Technology
雲雀野 : 豊橋技術科学大学人文科学系紀要 = The lark hill : bulletin of the School of Humanities, Toyohashi University of Technology (35), 47-55, 2013