Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination

• Suzuki, Daisuke

• Nagata, Toshi

• Matsumoto, Sohkichi

• Matsumoto, Makoto

• Tsujimura, Kunio

• Koide, Yukio

Abstract

application/pdf

Mycobacterial DNA-binding protein 1 (MDP1) is a major protein antigen in mycobacteria and induces protective immunity against Mycobacterium tuberculosis infection in mice. In this study we determined murine T-cell epitopes on MDP1 with MDP1 DNA immunization in mice. We analyzed interferon-γ production from the MDP1 DNA-immune splenocytes in response to 20-mer overlapping peptides covering MDP1 protein. We identified several CD4+ T-cell epitopes in three inbred mouse strains and one CD8+ T-cell epitope in C57BL/6 mice. These T-cell epitopes would be feasible for analysis of the role of MDP1-specific T-cells in protective immunity and for future vaccine design against M. tuberculosis infection.

Journal

• Vaccine

  Vaccine 28 (8), 2020-2025, 2010-02-23

  Elsevier

Keywords

• DNA immunization
• T-cell epitope
• Mycobacterium tuberculosis

Details

• CRID: 1050001201678761728
• NII Article ID: 120005305392
• ISSN: 0264410X
• HANDLE: 10271/2406
• Web Site: https://hama-med.repo.nii.ac.jp/records/2349
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles