Overexpression of the acerola (Malpighia glabra) monodehydroascorbate reductase gene in transgenic tobacco plants results in increased ascorbate levels and enhanced tolerance to salt stress

• Elteliba, Hani A

• Fujikawa, Yukichi

• Esaka, Muneharu

Abstract

Monodehydroascorbate reductase (MDHAR, EC 1.6.5.4) is a key enzyme of the ascorbate (AsA)-glutathione cycle that maintains reduced pools of AsA and serves as an important antioxidative enzyme. Previously, we have cloned MDHAR cDNA from acerola (Malpighia glabra), a plant that accumulates abundant amount of AsA. In this study, MDHAR cDNA from acerola was introduced into tobacco plants using an Agrobacterium-mediated gene delivery system. Transgenic tobacco plants accumulated greater amounts of AsA and showed higher MDHAR activity than the control plants. Lipid peroxidation and chlorophyll degradation, which were stimulated in control plants, were restrained in transgenic plants subjected to salt stress. These results indicate that overexpression of acerola MDHAR imparts greater tolerance to salt stress. (C) 2011 SAAB. Published by Elsevier B.V. All rights reserved.

Journal

• South African Journal of Botany

  South African Journal of Botany 78 295-301, 2012

  Elsevier Science BV

Keywords

• Acerola (Malpighia glabra)
• Ascorbic acid
• Monodehydroascorbate reductase
• Salt stress
• Transgenic

Details

• CRID: 1050014791008834304
• NII Article ID: 120005308220
• NII Book ID: AA10539383
• Web Site: http://ir.lib.hiroshima-u.ac.jp/00034799
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles