Generation of functional antibodies for mammalian membrane protein crystallography.

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Abstract

Membrane proteins act as gateways to cells, and they are responsible for much of the communication between cells and their environments. Crystallography of membrane proteins is often limited by the difficulty of crystallization in detergent micelles. Co-crystallization with antibody fragments has been reported as a method to facilitate the crystallization of membrane proteins; however, it is widely known that the generation of mouse monoclonal antibodies that recognize the conformational epitopes of mammalian integral membrane proteins is typically difficult. Here, we present our protocols to generate functional mouse antibodies for the membrane protein crystallography, which have enabled us to solve crystal structures of mammalian receptors and transporters complexed with antibody fragments.

Journal

  • Current opinion in structural biology

    Current opinion in structural biology 23(4), 563-568, 2013-08

    Elsevier Ltd.

Codes

  • NII Article ID (NAID)
    120005312201
  • NII NACSIS-CAT ID (NCID)
    AA10836185
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0959-440X
  • Data Source
    IR 
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