Generation of functional antibodies for mammalian membrane protein crystallography.

この論文にアクセスする

この論文をさがす

抄録

Membrane proteins act as gateways to cells, and they are responsible for much of the communication between cells and their environments. Crystallography of membrane proteins is often limited by the difficulty of crystallization in detergent micelles. Co-crystallization with antibody fragments has been reported as a method to facilitate the crystallization of membrane proteins; however, it is widely known that the generation of mouse monoclonal antibodies that recognize the conformational epitopes of mammalian integral membrane proteins is typically difficult. Here, we present our protocols to generate functional mouse antibodies for the membrane protein crystallography, which have enabled us to solve crystal structures of mammalian receptors and transporters complexed with antibody fragments.

収録刊行物

  • Current opinion in structural biology

    Current opinion in structural biology 23(4), 563-568, 2013-08

    Elsevier Ltd.

各種コード

  • NII論文ID(NAID)
    120005312201
  • NII書誌ID(NCID)
    AA10836185
  • 本文言語コード
    ENG
  • 資料種別
    journal article
  • ISSN
    0959-440X
  • データ提供元
    IR 
ページトップへ