LIQUID CHROMATOGRAPHIC / MASS SPECTROMETRIC DETERMINATION OF MORPHINE, CODEINE AND COCAINE IN HUMAN SERA USING A NEW INTERNAL SURFACE REVERSED PHASE COLUMN

• Hattori, Hideki

• Arinobu, Tetsuya

• Iwai, Masae

• Suzuki, Osamu

• Seno, Hiroshi

Bibliographic Information

Other Title

• 新内面逆相ODSカラムを用いた血清中モルヒネ，コデインならびにコカインのLS/MSによる定量

Abstract

A new internal surface reversed phase column (Shim-pack MAYI-ODS) was used for analysis of morphine, codeine and cocaine in human sera by LC/MS/MS. The column enabled direct injection of crude biological samples without pretreatment realizing a rapid analytical procedure. The recoveries of morphine, codeine and cocaine spiked into human sera were 50-60 %. The regression equations for morphine, codeine and cocaine in sera, using atropine as internal standard, showed good linearity in the ranges of 100-500. 50-250 and 20-100 ng/ml, respectively; the detection limits were 50, 20 and 10 ng/ml, respectively. The coefficients of intra-day variation for the three compounds spiked into sera were less than 7 %. The proposed method seems useful for toxicological analysis of three drugs, because of rapidness and reproducibility.

Journal

• Japanese journal of forensic toxicology

  Japanese journal of forensic toxicology 21 (1), 47-54, 2003-01-31

  日本法中毒学会

Keywords

• Morphine
• Codeine
• Cocaine
• Atropine
• New internal surface reversed phase column
• Shim-pack MAYI-ODS
• LC/MS/MS

Details

• CRID: 1050001201673849728
• NII Article ID: 120005314056
• ISSN: 09159606
• HANDLE: 10271/1740
• Web Site: https://hama-med.repo.nii.ac.jp/records/1707
• Web Site: https://search.jamas.or.jp/link/ui/2003223750
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles