MANGANESE LEVELS IN TISSUES OF PARAQUAT DOSED AND/OR MAGNESIUM RESTRICTED RATS MEASURED BY AN ELECTRON SPIN RESONANCE METHOD

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  • ESR測定によるパラコート投与およびマグネシウム制限食投与ラットの組織中マンガン濃度

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Abstract

The effects of drugs and nutritions on tissue manganese (Mn) levels have not been well studied, because of the quite low levels of Mn in mammalian tissues. Furthermore, Mn-complexes with chelators are so unstable that colorimetric assays of the Mncomplexes are interfered with by metal ions commonly present in tissues. To measure such trace amounts of Mn, we have used an electron spin resonance (ESR) method, since Mn2 + is paramagnetic. By this method, Mn2 + could be quantitated using 5μl of its solution at 100 ppb with the error of 5%. The effects of paraquat dosing and/or magnesium (Mg) restriction in the diets for 8 days on tissue Mn and Mg levels were studied using osteogenic disorder Shionogi rats, a mutant strain which cannot synthesize vitamin C like humans. Tissue Mn levels of the animals were not affected by either dosing with paraquat at 125 ppm or Mg restriction at half of that recommended by American Institute of Nutrition-93. When both treatments were given together, however, Mn levels were significantly lowered in the liver, kidney and heart. Mn levels of rats dosed with paraquat at 250 ppm were more significantly lowered in all tissues studied.

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