RAPID DETERMINATION OF THIOCYANATE IN BIOLOGICAL FLUIDS BY USE OF ELECTRON SPIN RESONANCE

• Minakata, Kayoko

• Nozawa, Hideki

• Watanabe, Kanako

• Suzuki, Osamu

Bibliographic Information

Other Title

• 電子スピン共鳴法を用いたチオシアン酸イオンの迅速定量

Abstract

A simple and sensitive method has been developed for determination of thiocyanate ion (SCN-), the main metabolite of cyanide, in biological fluids and beverages. The procedure was based on the formation of a paramagnetic complex Cu(SCN）2(4-methylpyridine）2 in benzene, followed by its detection by electron spin resonance (ESR). The complex showed a characteristic four-peak hyperfine structure with a g-value of 2.132 at room temperature. By the present method, SCN- at concentrations from 5 μM to 2 mM in 500 μl solution could be determined. The recoveries were more than 95% for SCN- added to blood, plasma, urine, milk and beverages at 200 μM. Several anions and cations in biological fluids did not interfere with the formation of the complex in the presence of large amounts of Cu2+. Our method is recommendable, because it takes only less than 10 min to determine SCN- and the produced complex is quite stable.

Journal

• Japanese journal of forensic toxicology

  Japanese journal of forensic toxicology 23 (1), 41-44, 2005-01-31

  日本法中毒学会

Keywords

• Thiocyanate
• Cyanide metabolite
• Electron spin resonance
• Copper
• Blood
• Urine

Details

• CRID: 1050001201678721024
• NII Article ID: 120005314068
• ISSN: 09159606
• HANDLE: 10271/1752
• Web Site: https://hama-med.repo.nii.ac.jp/records/1719
• Web Site: https://search.jamas.or.jp/link/ui/2006137588
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN