Determination of cyanide, in urine and gastric content, by electrospray ionization tandem mass spectrometry after direct flow injection of dicyanogold

• Minakata, Kayoko

• Nozawa, Hideki

• Gonmori, Kunio

• Suzuki, Masako

• Suzuki, Osamu

Abstract

A rapid and sensitive electrospray ionization tandem mass spectrometric (ESI-MS-MS) procedure was developed for the determination of cyanide (CN-). CN- in biological fluids was reacted with NaAuCl4 to produce dicyanogold, Au(CN)2- which was extracted with methyl isobutyl ketone (MIBK). One μL of the extract was injected directly into an ESI-MS-MS instrument. Quantification of CN-was performed by selected reaction monitoring of the product ion CN- at m/z 26 that derived from precursor ion Au(CN)2- at m/z 249. CN- could be measured in the quantification range of 10-7 to 5×10-5 M with the limit of detection at 4×10-8 M using 10 μL of urine within 10 min. A victim’s urine and gastric content were diluted with water to 4-fold and 500-fold and measured, respectively.

Journal

• Analytica Chimica Acta

  Analytica Chimica Acta 651 (1), 81-84, 2009-09-28

  Elsevier

Keywords

• Cyanide
• Gold
• Electrospray ionization
• Tandem mass spectrometry
• Urine

Details

• CRID: 1050282676643412608
• NII Article ID: 120005314222
• ISSN: 00032670
• HANDLE: 10271/1920
• Web Site: https://hama-med.repo.nii.ac.jp/records/1883
• Text Lang: en
• Article Type: journal article
• Data Source:
  • IRDB
  • CiNii Articles
  • KAKEN