SIMPLE AND RAPID ASSAY METHOD FOR SIMULTANEOUS QUANTIFICATION OF URINARY NICOTINE AND COTININE USING MICRO-EXTRACTION BY PACKED SORBENT AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY

• Iwai Masae

• Ogawa Tadashi

• Hattori Hideki

• Zaitsu Kei

• Ishii Akira

• Suzuki Osamu

• Seno Hiroshi

Abstract

A simple and rapid method for determination of nicotine and cotinine levels in urine was developed using samples prepared by micro-extraction by packed sorbent (MEPS) and subjected to gas chromatography- mass spectrometry (GC-MS) analysis. This method provided good reproducibility, as well as good linearity of calibration curves in the range of 1–100 and 50–1000 ng/mL for quality control samples spiked with nicotine and cotinine, respectively. The detection limit of nicotine and cotinine was as low as 0.25 and 20 ng/mL, respectively. An evaporation procedure is not suitable for nicotine determination, thus an advantage of the present MEPS assay method is direct testing with GC-MS without the need for evaporation to a dry solvent. Our findings show that it may be useful for determining nicotine levels in various types of research studies.

Journal

• Nagoya Journal of Medical Science

  Nagoya Journal of Medical Science 75 (3-4), 255-261, 2013-08

  Nagoya University School of Medicine

Keywords

• micro-extraction by packed sorbent (MEPS)
• gas chromatography-mass spectrometry (GC-MS)
• nicotine
• cotinine

Details

• CRID: 1390009224622971392
• NII Article ID: 120005322406
• DOI: 10.18999/nagjms.75.3-4.255
• ISSN: 21863326
• ISSN: 00277622
• HANDLE: 2237/18475
• Web Site: https://nagoya.repo.nii.ac.jp/records/16458
• Web Site: https://search.jamas.or.jp/link/ui/2014280759
• Text Lang: en
• Data Source:
  • JaLC
  • IRDB
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Allowed