Cooperative Synthesis of Ultra Long-Chain Fatty Acid and Ceramide during Keratinocyte Differentiation

• Mizutani, Yukiko

• Sun, Hui

• Ohno, Yusuke

• Sassa, Takayuki

• Wakashima, Takeshi

• Obara, Mari

• Yuyama, Kohei

• Kihara, Akio

• Igarashi, Yasuyuki

抄録

The lipid lamellae in the stratum corneum is important for the epidermal permeability barrier. The lipid lamellae component ceramide (CER), comprising an ultra long-chain (ULC) fatty acid (FA) of >= 26 carbons (ULC CER), plays an essential role in barrier formation. ULC acyl-CoAs, produced by the FA elongase ELOVL4, are converted to ULC CERs by the CER synthase CERS3. In the presented study, we observed that ELOVL4 and CERS3 mRNAs increased during keratinocyte differentiation in vivo and in vitro. We also determined that peroxisome proliferator-activated receptor beta/delta is involved in the up-regulation of the mRNAs. Knockdown of CERS3 caused a reduction in the elongase activities toward ULC acyl-CoAs, suggesting that CERS3 positively regulates ULCFA. Thus, we reveal that the two key players in ULC CER production in epidermis, CERS3 and ELOVL4, are coordinately regulated at both the transcriptional and enzymatic levels.

収録刊行物

• Plos One

  Plos One 8 (6), e67317-, 2013-06-27

  Public Library of Science

詳細情報

• CRID: 1050564288967847936
• NII論文ID: 120005322424
• HANDLE: 2115/53154
• ISSN: 19326203
• 本文言語コード: en
• 資料種別: journal article
• データソース種別:
  • IRDB
  • CiNii Articles