Direct Induction of Chondrogenic Cells from Human Dermal Fibroblast Culture by Defined Factors

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Abstract

ヒトの皮膚細胞から軟骨様細胞へ直接変換に成功. 京都大学プレスリリース. 2013-10-14.

The repair of large cartilage defects with hyaline cartilage continues to be a challenging clinical issue. We recently reported that the forced expression of two reprogramming factors (c-Myc and Klf4) and one chondrogenic factor (SOX9) can induce chondrogenic cells from mouse dermal fibroblast culture without going through a pluripotent state. We here generated induced chondrogenic (iChon) cells from human dermal fibroblast (HDF) culture with the same factors. We developed a chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells. The human iChon cells expressed marker genes for chondrocytes but not fibroblasts, and were derived from non-chondrogenic COL11A2-negative cells. The human iChon cells formed cartilage but not tumors in nude mice. This approach could lead to the preparation of cartilage directly from skin in human, without going through pluripotent stem cells.

Journal

  • PLoS ONE

    PLoS ONE 8(10), 2013-10-16

    Public Library of Science

Codes

  • NII Article ID (NAID)
    120005333915
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    1932-6203
  • Data Source
    IR 
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